Repair of UVB-induced DNA damage is reduced in melanoma due to low XPC and global genome repair.

ABSTRACT: UVB exposure leads to DNA damage, which when unrepaired induces C>T transitions. These mutations are found throughout the melanoma genome, particularly in non-transcribed regions. The global genome repair (GGR) branch of nucleotide excision repair (NER) is responsible for repairing UV-induced DNA damage across non-transcribed and silent regions of the genome. This study aimed to examine the relationship between UVB and GGR in melanoma. DNA repair capacity and relative expression of NER in melanocytes and melanoma cell lines before and after treatment with UVB was quantified. Transcript expression from 196 melanomas was compared to clinical parameters including solar elastosis and whole transcriptome data collected. Melanoma cell lines showed significantly reduced DNA repair when compared t

SUBMITTER: Budden T 

PROVIDER: S-EPMC5308628 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature