Human astrocytes secrete IL-6 to promote glioma migration and invasion through upregulation of cytomembrane MMP14.

ABSTRACT: The brain microenvironment has emerged as an important component in malignant progression of human glioma. However, astrocytes, the most abundant glial cells in the glioma microenvironment, have as yet a poorly defined role in the development of this disease, particularly with regard to invasion. Here, we co-cultured human astrocytes with human glioma cell lines, U251 and A172, in an in vitro transwell system in order to ascertain their influence on migration and invasion of gliomas. mRNA and protein expression assays were subsequently used to identify candidate proteins mediating this activity. Astrocytes significantly increased migration and invasion of both U251 and A172 cells in migration and invasion (plus matrigel) assays. Membrane type 1 matrix metalloproteinase (MMP14) originating

SUBMITTER: Chen W 

PROVIDER: S-EPMC5308737 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature