Actin dynamics and competition for myosin monomer govern the sequential amplification of myosin filaments.

ABSTRACT: The cellular mechanisms governing non-muscle myosin II (NM2) filament assembly are largely unknown. Using EGFP-NM2A knock-in fibroblasts and multiple super-resolution imaging modalities, we characterized and quantified the sequential amplification of NM2 filaments within lamellae, wherein filaments emanating from single nucleation events continuously partition, forming filament clusters that populate large-scale actomyosin structures deeper in the cell. Individual partitioning events coincide spatially and temporally with the movements of diverging actin fibres, suppression of which inhibits partitioning. These and other data indicate that NM2A filaments are partitioned by the dynamic movements of actin fibres to which they are bound. Finally, we showed that partition frequency and filamen

SUBMITTER: Beach JR 

PROVIDER: S-EPMC5308804 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature