Transient Opening of the Mitochondrial Permeability Transition Pore Induces Microdomain Calcium Transients in Astrocyte Processes.

ABSTRACT: Astrocytes extend highly branched processes that form functionally isolated microdomains, facilitating local homeostasis by redistributing ions, removing neurotransmitters, and releasing factors to influence blood flow and neuronal activity. Microdomains exhibit spontaneous increases in calcium (Ca²⁺), but the mechanisms and functional significance of this localized signaling are unknown. By developing conditional, membrane-anchored GCaMP3 mice, we found that microdomain activity that occurs in the absence of inositol triphosphate (IP3)-dependent release from endoplasmic reticulum arises through Ca²⁺ efflux from mitochondria during brief openings of the mitochondrial permeability transition pore. These microdomain Ca²⁺ transients were facilitated by the pro

SUBMITTER: Agarwal A 

PROVIDER: S-EPMC5308886 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature