The role and possible mechanism of lncRNA U90926 in modulating 3T3-L1 preadipocyte differentiation.

ABSTRACT:

Background

Obesity is a risk factor for metabolic diseases, while preadipocyte differentiation or adipogenesis is closely related to obesity occurrence. Long noncoding RNAs (lncRNAs) are a unique class of transcripts in regulation of a variety of biological processes. Using cDNA microarray, we found lncRNA U90926 is negatively correlated with 3T3-L1 preadipocyte differentiation.

Objective

The aim of this study was to explore the role of lncRNA U90926 (lnc-U90926) in adipogenesis and the underlying mechanisms.

Methods

Quantitative real-time PCR (qPCR) was performed to determine lnc-U90926 expression in 3T3-L1 preadipocytes, differentiated adipocytes, and in adipose tissues form mice. RNA fluorescent in situ hybridization (FISH) was performed to determine the localizati

SUBMITTER: Chen J 

PROVIDER: S-EPMC5309343 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature