Effect of tcdR Mutation on Sporulation in the Epidemic Clostridium difficile Strain R20291.

ABSTRACT: Clostridium difficile is an important nosocomial pathogen and the leading cause of hospital-acquired diarrhea. Antibiotic use is the primary risk factor for the development of C. difficile-associated disease because it disrupts normally protective gut flora and enables C. difficile to colonize the colon. C. difficile damages host tissue by secreting toxins and disseminates by forming spores. The toxin-encoding genes, tcdA and tcdB, are part of a pathogenicity locus, which also includes the tcdR gene that codes for TcdR, an alternate sigma factor that initiates transcription of tcdA and tcdB genes. We created a tcdR mutant in epidemic-type C. difficile strain R20291 in an attempt to identify the global role of t

SUBMITTER: Girinathan BP 

PROVIDER: S-EPMC5311115 | biostudies-literature | 2017 Jan-Feb

REPOSITORIES: biostudies-literature