Genome Integration and Excision by a New Streptomyces Bacteriophage, ϕJoe.

ABSTRACT: Bacteriophages are the source of many valuable tools for molecular biology and genetic manipulation. In Streptomyces, most DNA cloning vectors are based on serine integrase site-specific DNA recombination systems derived from phage. Because of their efficiency and simplicity, serine integrases are also used for diverse synthetic biology applications. Here, we present the genome of a new Streptomyces phage, ϕJoe, and investigate the conditions for integration and excision of the ϕJoe genome. ϕJoe belongs to the largest Streptomyces phage cluster (R4-like) and encodes a serine integrase. The attB site from Streptomyces venezuelae was used efficiently by an integrating plasmid, pCMF92, constructed using the ϕJoe int-attP locus. The attB site fo

SUBMITTER: Fogg PCM 

PROVIDER: S-EPMC5311408 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature