Viral E6 is overexpressed via high viral load in invasive cervical cancer with episomal HPV16.

ABSTRACT:

Background

The integration of HR-HPV genome into host DNA is regarded as a key step for the development of cervical cancer. However, HR-HPV genome indeed exists as episome except for integrant. It may be alternative mechanisms in episome-associated carcinogenesis, although, by which HPV 16 episome induces cervical carcinogenesis is unclear now.

Methods

Ninety-three invasive cervical cancer tissues with HPV16 positive were collected. Viral physical status was calculated from comparing E2 to E6-copies and detection of viral load was made with realtime-PCR using copy numbers of E6. HPV16 E6 mRNA transcript levels were measured by realtime-PCR. The methylation frequency of HPV16 promoter was detected by PCR and pyrosequencing.

Results

In 93 samples, 21.5% (20/93) presente

SUBMITTER: Hong D 

PROVIDER: S-EPMC5311840 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature