Improvement of peptide identification with considering the abundance of mRNA and peptide.

ABSTRACT:

Background

Tandem mass spectrometry (MS/MS) followed by database search is a main approach to identify peptides/proteins in proteomic studies. A lot of effort has been devoted to improve the identification accuracy and sensitivity for peptides/proteins, such as developing advanced algorithms and expanding protein databases.

Results

Herein, we described a new strategy for enhancing the sensitivity of protein/peptide identification through combination of mRNA and peptide abundance in Percolator. In our strategy, a new workflow for peptide identification is established on the basis of the abundance of transcripts and potential novel transcripts derived from RNA-Seq and abundance of peptides towards the same life species. We demonstrate the utility of this strategy by two MS/MS

SUBMITTER: Ma C 

PROVIDER: S-EPMC5311845 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature