R-spondin 1 is required for specification of hematopoietic stem cells through Wnt16 and Vegfa signaling pathways.

ABSTRACT: Hematopoietic stem cells (HSCs) are the therapeutic component of bone marrow transplants, but finding immune-compatible donors limits treatment availability and efficacy. Recapitulation of endogenous specification during development is a promising approach to directing HSC specification in vitro, but current protocols are not capable of generating authentic HSCs with high efficiency. Across phyla, HSCs arise from hemogenic endothelium in the ventral floor of the dorsal aorta concurrent with arteriovenous specification and intersegmental vessel (ISV) sprouting, processes regulated by Notch and Wnt. We hypothesized that coordination of HSC specification with vessel patterning might involve modulatory regulatory factors such as R-spondin 1 (Rspo1), an extracellular protein that enhance

SUBMITTER: Genthe JR 

PROVIDER: S-EPMC5312032 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature