Proteomic identification of cyclophilin A as a potential biomarker and therapeutic target in oral submucous fibrosis.

ABSTRACT: Oral submucous fibrosis (OSF) is a pre-cancerous lesion, which is characterized by fibrosis of the oral submucosa. Despite large body of studies focusing on this disease, the molecular mechanisms underlying the progression of OSF remained unclear. In this study, 2-DE-based proteomic approaches were employed to identify the differently expressed proteins between OSF and normal tissues. In total, 88 proteins were identified with altered expression levels, including CypA. Upregulation of CypA was further validated through immunohistochemistry staining combined with Q-PCR and western blot by using clinical samples. Statistical analyses reveal that CypA expression level is correlated to the progression of OSF. Finally, functional study reveals a pro-proliferative property of CypA in fibroblast

SUBMITTER: Yuan Y 

PROVIDER: S-EPMC5312388 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature