Establishment of national reference for bunyavirus nucleic acid detection kits for diagnosis of SFTS virus.

ABSTRACT:

Background

Severe fever with thrombocytopenia syndrome (SFTS) caused by SFTS virus (SFTSV) usually have a high fatality. At present no effective therapy or vaccine are available, so early diagnosis of SFTS is crucial to prevent and control SFTSV infection. This study aimed to establish a national reference for these diagnostic kits of SFTSV genome and make the diagnosis of the disease effective.

Methods

Six SFTSV strains isolated from different regions, and five relative viruses with similar clinical manifestations were selected as positive and negative references and assessed using real time quantitative PCR (q-PCR) using specific primers and probe and two commercial kits. The stability of the references was also assessed at 37 °C, room temperature or -70 °C for 8 days, 14 

SUBMITTER: Lu X 

PROVIDER: S-EPMC5312432 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature