DNA barcoding of selected UAE medicinal plant species: a comparative assessment of herbarium and fresh samples.

ABSTRACT: It is commonly difficult to extract and amplify DNA from herbarium samples as they are old and preserved using different compounds. In addition, such samples are subjected to the accumulation of intrinsically produced plant substances over long periods (up to hundreds of years). DNA extraction from desert flora may pause added difficulties as many contain high levels of secondary metabolites. Herbarium samples from the Biology Department (UAE University) plant collection and fresh plant samples, collected from around Al-Ain (UAE), were used in this study. The three barcode loci for the coding genes matK, rbcL and rpoC1-were amplified. Our results showed that T. terresteris, H. robustum,T. pentandrus and Z. qatarense were amplified using all three primers for bot

SUBMITTER: Enan MR 

PROVIDER: S-EPMC5313416 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature