Defining the role of corticotropin releasing factor binding protein in alcohol consumption.

ABSTRACT: The corticotropin releasing factor (CRF) exerts its effects by acting on its receptors and on the binding protein (CRFBP), and has been implicated in alcohol use disorder (AUD). Therefore, identification of the exact contribution of each protein that mediates CRF effects is necessary to design effective therapeutic strategies for AUD. A series of in vitro/in vivo experiments across different species were performed to define the biological discrete role of CRFBP in AUD. First, to establish the CRFBP role in receptor signaling, we developed a novel chimeric cell-based assay and showed that CFRBP full length can stably be expressed on the plasma membrane. We discovered that only CRFBP(10 kD) fragment is able to potentiate CRF-intracellular Ca²⁺ release. We provide evidence that CRH

SUBMITTER: Haass-Koffler CL 

PROVIDER: S-EPMC5314120 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature