Prognostic significance of vascular endothelial growth factor polymorphisms in colorectal cancer patients.

ABSTRACT:

Aim

To investigate the associations of the genetic polymorphisms of vascular endothelial growth factor A (VEGF-A) -1498C>T and -634G>C, with the survival of patients with colorectal cancer (CRC).

Methods

A prospective cohort consisting of 131 Brazilians patients consecutively operated on with a curative intention as a result of sporadic colorectal carcinoma was studied. DNA was extracted from peripheral blood and its amplification and allelic discrimination for each genetic polymorphism was performed using the technique of polymerase chain reaction (PCR) in real-time. The real-time PCR technique was used to identify the VEGF-A -1498C>T (rs833031) and -634G>C (rs2010963) polymorphisms. Genotyping was validated for VEGF-A -1498C>T polymorphism in 129 patie

SUBMITTER: do Espirito Santo GF 

PROVIDER: S-EPMC5314204 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature