High-resolution 3D imaging of whole organ after clearing: taking a new look at the zebrafish testis.

ABSTRACT: Zebrafish testis has become a powerful model for reproductive biology of teleostean fishes and other vertebrates and encompasses multiple applications in applied and basic research. Many studies have focused on 2D images, which is time consuming and implies extrapolation of results. Three-dimensional imaging of whole organs recently became an important challenge to better understand their architecture and allow cell enumeration. Several protocols have thus been developed to enhance sample transparency, a limiting step for imaging large biological samples. However, none of these methods has been applied to the zebrafish testis. We tested five clearing protocols to determine if some of them could be applied with only small modifications to the testis. We compared clearing efficiency at both

SUBMITTER: Fretaud M 

PROVIDER: S-EPMC5314416 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature