Unaltered Glutamate Transporter-1 Protein Levels in Aquaporin-4 Knockout Mice.

ABSTRACT: Maintenance of glutamate and water homeostasis in the brain is crucial to healthy brain activity. Astrocytic glutamate transporter-1 (GLT1) and aquaporin-4 (AQP4) are the main regulators of extracellular glutamate and osmolarity, respectively. Several studies have reported colocalization of GLT1 and AQP4, but the existence of a physical interaction between the two has not been well studied. Therefore, we used coimmunoprecipitation to determine whether a strong interaction exists between these two important molecules in mice on both a CD1 and C57BL/6 background. Furthermore, we used Western blot and immunohistochemistry to examine GLT1 levels in AQP4 knockout (AQP4^-/-) mice. An AQP4-GLT1 precipitate was not detected, suggesting the lack of a strong physical interaction between AQ

SUBMITTER: Hubbard JA 

PROVIDER: S-EPMC5315234 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature