Evaluation and comparison of FTA card and CTAB DNA extraction methods for non-agricultural taxa.

ABSTRACT: An efficient, effective DNA extraction method is necessary for comprehensive analysis of plant genomes. This study analyzed the quality of DNA obtained using paper FTA cards prepared directly in the field when compared to the more traditional cetyltrimethylammonium bromide (CTAB)-based extraction methods from silica-dried samples. DNA was extracted using FTA cards according to the manufacturer's protocol. In parallel, CTAB-based extractions were done using the automated AutoGen DNA isolation system. DNA quality for both methods was determined for 15 non-agricultural species collected in situ, by gel separation, spectrophotometry, fluorometry, and successful amplification and sequencing of nuclear and chloroplast gene markers. The FTA card extraction method yielded less concentrated, but al

SUBMITTER: Siegel CS 

PROVIDER: S-EPMC5315379 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature