Genetically encoded ratiometric fluorescent thermometer with wide range and rapid response.

ABSTRACT: Temperature is a fundamental physical parameter that plays an important role in biological reactions and events. Although thermometers developed previously have been used to investigate several important phenomena, such as heterogeneous temperature distribution in a single living cell and heat generation in mitochondria, the development of a thermometer with a sensitivity over a wide temperature range and rapid response is still desired to quantify temperature change in not only homeotherms but also poikilotherms from the cellular level to in vivo. To overcome the weaknesses of the conventional thermometers, such as a limitation of applicable species and a low temporal resolution, owing to the narrow temperature range of sensitivity and the thermometry method, respectively, we developed a

SUBMITTER: Nakano M 

PROVIDER: S-EPMC5315395 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature