Improving acetyl-CoA biosynthesis in Saccharomyces cerevisiae via the overexpression of pantothenate kinase and PDH bypass.

ABSTRACT:

Background

Acetyl-CoA is an important precursor in Saccharomyces cerevisiae. Various approaches have been adopted to improve its cytosolic level previously with the emphasis on engineering the "acetyl-" part of acetyl-CoA. To the best of our knowledge, there have been no reports on engineering the "-CoA" part so far.

Results

In this study, we had tried to engineer S. cerevisiae from both the "-CoA" part via pantothenate kinase overexpression (PanK from S. cerevisiae, the rate-limiting enzyme for CoA synthesis) and the "acetyl-"part through PDH bypass introduction (ALD6 from S. cerevisiae and SeAcs^L641P from Salmonella enteric). A naringenin-producing reporter strain had been constructed to reflect cytosolic a

SUBMITTER: Liu W 

PROVIDER: S-EPMC5316175 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature