Che-1 sustains hypoxic response of colorectal cancer cells by affecting Hif-1α stabilization.

ABSTRACT:

Background

Solid tumours are less oxygenated than normal tissues. Consequently, cancer cells acquire to be adapted to a hypoxic environment. The poor oxygenation of solid tumours is also a major indicator of an adverse cancer prognosis and leads to resistance to conventional anticancer treatments. We previously showed the involvement of Che-1/AATF (Che-1) in cancer cell survival under stress conditions. Herein we hypothesized that Che-1 plays a role in the response of cancer cells to hypoxia.

Methods

The human colon adenocarcinoma HCT116 and HT29 cell lines undepleted or depleted for Che-1 expression by siRNA, were treated under normoxic and hypoxic conditions to perform studies regarding the role of this protein in metabolic adaptation and cell proliferation. Che-1 expressi

SUBMITTER: Bruno T 

PROVIDER: S-EPMC5316229 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature