Role of protein kinase C in metabolic regulation of the cardiac Na⁺ channel.

ABSTRACT:

Background

The reduced form of nicotinamide adenine dinucleotide (NADH) increases in cardiomyopathy, activates protein kinase C (PKC), up-regulates mitochondrial reactive oxygen species (mitoROS), and down-regulates the cardiac Na⁺ channel (Na_V1.5).

Objective

The purpose of this study was to determine how NADH signals down-regulation of Na_V1.5.

Methods

Isolated mouse cardiomyocytes were used for patch-clamp recording and for monitoring mitoROS with MitoSOX Red. HEK293 cells were used for transient transfections. HEK293 cells stably expressing human Na_V1.5 were used for single channel recording, whole-cell patch-clamp recording, activity measurements of phospholipase C and phospholipase D (PLD), channel protein purification,

SUBMITTER: Liu M 

PROVIDER: S-EPMC5316355 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature