Broadening of analyte streams due to a transverse pressure gradient in free-flow isoelectric focusing.

ABSTRACT: Pressure-driven cross-flows can arise in free-flow isoelectric focusing systems (FFIEF) due to a non-uniform electroosmotic flow velocity along the channel width induced by the pH gradient in this direction. In addition, variations in the channel cross-section as well as unwanted differences in hydrostatic heads at the buffer/sample inlet ports can also lead to such pressure-gradients which besides altering the equilibrium position of the sample zones have a tendency to substantially broaden their widths deteriorating the separations. In this situation, a thorough assessment of stream broadening due to transverse pressure-gradients in FFIEF devices is necessary in order to establish accurate design rules for the assay. The present article describes a mathematical framework to estimate the

SUBMITTER: Dutta D 

PROVIDER: S-EPMC5316482 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature