STIM1 Phosphorylation at Y361 Recruits Orai1 to STIM1 Puncta and Induces Ca²⁺ Entry.

ABSTRACT: Store-operated Ca²⁺ entry (SOCE) mediates the increase in intracellular calcium (Ca²⁺) in endothelial cells (ECs) that regulates several EC functions including tissue-fluid homeostasis. Stromal-interaction molecule 1 (STIM1), upon sensing the depletion of (Ca²⁺) from the endoplasmic reticulum (ER) store, organizes as puncta that trigger store-operated Ca²⁺ entry (SOCE) via plasmalemmal Ca²⁺-selective Orai1 channels. While the STIM1 and Orai1 binding interfaces have been mapped, signaling mechanisms activating STIM1 recruitment of Orai1 and STIM1-Orai1 interaction remains enigmatic. Here, we show that ER Ca²⁺-store depletion rapidly induces STIM1 phosphorylation at Y361 via proline-rich kinase 2 (Pyk2) in ECs. Surprisingly, t

SUBMITTER: Yazbeck P 

PROVIDER: S-EPMC5316956 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature