Interleukin 10 inhibits pro-inflammatory cytokine responses and killing of Burkholderia pseudomallei.

ABSTRACT: Melioidosis, caused by Burkholderia pseudomallei, is endemic in northeastern Thailand and Northern Australia. Severe septicemic melioidosis is associated with high levels of pro-inflammatory cytokines and is correlated with poor clinical outcomes. IL-10 is an immunoregulatory cytokine, which in other infections can control the expression of pro-inflammatory cytokines, but its role in melioidosis has not been addressed. Here, whole blood of healthy seropositive individuals (n = 75), living in N. E. Thailand was co-cultured with B. pseudomallei and production of IL-10 and IFN-γ detected and the cellular sources identified. CD3^- CD14⁺ monocytes were the main source of IL-10. Neutralization of IL-10 increased IFN-γ, IL-6 and TNF-α production and improved bacteria killing.

SUBMITTER: Kessler B 

PROVIDER: S-EPMC5316963 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature