Plasma Exosome Profiling of Cancer Patients by a Next Generation Systems Biology Approach.

ABSTRACT: Technologies capable of characterizing the full breadth of cellular systems need to be able to measure millions of proteins, isoforms, and complexes simultaneously. We describe an approach that fulfils this criterion: Adaptive Dynamic Artificial Poly-ligand Targeting (ADAPT). ADAPT employs an enriched library of single-stranded oligodeoxynucleotides (ssODNs) to profile complex biological samples, thus achieving an unprecedented coverage of system-wide, native biomolecules. We used ADAPT as a highly specific profiling tool that distinguishes women with or without breast cancer based on circulating exosomes in their blood. To develop ADAPT, we enriched a library of ~10¹¹ ssODNs for those associating with exosomes from breast cancer patients or controls. The resulting 10⁶

SUBMITTER: Domenyuk V 

PROVIDER: S-EPMC5316983 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature