The Draft Genome and Transcriptome of the Atlantic Horseshoe Crab, Limulus polyphemus.

ABSTRACT: The horseshoe crab, Limulus polyphemus, exhibits robust circadian and circatidal rhythms, but little is known about the molecular mechanisms underlying those rhythms. In this study, horseshoe crabs were collected during the day and night as well as high and low tides, and their muscle and central nervous system tissues were processed for genome and transcriptome sequencing, respectively. The genome assembly resulted in 7.4 × 10⁵ contigs with N50 of 4,736, while the transcriptome assembly resulted in 9.3 × 10⁴ contigs and N50 of 3,497. Analysis of functional completeness by the identification of putative universal orthologs suggests that the transcriptome has three times more total expected orthologs than the genome. Interestingly, RNA-Seq analysis indicated no

SUBMITTER: Simpson SD 

PROVIDER: S-EPMC5317147 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature