Regulatory B Cells in Seropositive Myasthenia Gravis versus Healthy Controls.

ABSTRACT:

Objective

To find out if the failure in immunotolerance of myasthenia gravis (MG) is a possible aspect of deduction in Breg cells and to characterize B cell subsets in MG.

Methods

Flow cytometry detection and enzyme-linked immunosorbent assays in peripheral blood films of 10 MG patients and 10 healthy controls (HCs) were performed after isolation of B cells. The CD19⁺CD5⁺CD1d⁺ Breg cells percentages were measured to complement a B cell phenotype assay and frequencies of B cell subsets. The clinical outcome measures and immunological variables of patients with MG were compared with HCs.

Results

Patients with MG had relatively lowered percentages of CD19⁺CD5⁺CD1d⁺ Breg cells as compared to HCs. The produc

SUBMITTER: Karim MR 

PROVIDER: S-EPMC5317198 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature