Molecular typing for blood group antigens within 40 min by direct polymerase chain reaction from plasma or serum.

ABSTRACT: Determining blood group antigens by serological methods may be unreliable in certain situations, such as in patients after chronic or massive transfusion. Red cell genotyping offers a complementary approach, but current methods may take much longer than conventional serological typing, limiting their utility in urgent situations. To narrow this gap, we devised a rapid method using direct polymerase chain reaction (PCR) amplification while avoiding the DNA extraction step. DNA was amplified by PCR directly from plasma or serum of blood donors followed by a melting curve analysis in a capillary rapid-cycle PCR assay. We evaluated the single nucleotide polymorphisms underlying the clinically relevant Fy^a , Fy^b , Jk^a and Jk^b antigens, with our analys

SUBMITTER: Wagner FF 

PROVIDER: S-EPMC5318227 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature