Functional Diversity of Transcriptional Regulators in the Cyanobacterium Synechocystis sp. PCC 6803.

ABSTRACT: Functions of transcriptional regulators (TRs) are still poorly understood in the model cyanobacterium Synechocystis sp. PCC 6803. To address the issue, we constructed knockout mutants for 32 putative TR-encoding genes of Synechocystis, and comparatively analyzed their phenotypes under autotrophic growth condition and metabolic profiles using liquid chromatography-mass spectrometry-based metabolomics. The results showed that only four mutants of TR genes, sll1872 (lytR), slr0741 (phoU), slr0395 (ntcB), and slr1871 (pirR), showed differential growth patterns in BG11 medium when compared with the wild type; however, in spite of no growth difference observed for the remaining TR mutants, metabolomic profiling showed that the

SUBMITTER: Shi M 

PROVIDER: S-EPMC5318462 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature