Flow Dynamics and HSPC Homing in Bone Marrow Microvessels.

ABSTRACT: Measurements of flow velocities at the level of individual arterial vessels and sinusoidal capillaries are crucial for understanding the dynamics of hematopoietic stem and progenitor cell homing in the bone marrow vasculature. We have developed two complementary intravital two-photon imaging approaches to determine blood flow dynamics and velocities in multiple vessel segments by capturing the motion of red blood cells. High-resolution spatiotemporal measurements through a cranial window to determine short-time dynamics of flowing blood cells and repetitive centerline scans were used to obtain a detailed flow-profile map with hemodynamic parameters. In addition, we observed the homing of individual hematopoietic stem and progenitor cells and obtained detailed information on their homing be

SUBMITTER: Bixel MG 

PROVIDER: S-EPMC5318670 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature