Image processing in digital pathology: an opportunity to solve inter-batch variability of immunohistochemical staining.

ABSTRACT: Immunohistochemistry (IHC) is a widely used technique in pathology to evidence protein expression in tissue samples. However, this staining technique is known for presenting inter-batch variations. Whole slide imaging in digital pathology offers a possibility to overcome this problem by means of image normalisation techniques. In the present paper we propose a methodology to objectively evaluate the need of image normalisation and to identify the best way to perform it. This methodology uses tissue microarray (TMA) materials and statistical analyses to evidence the possible variations occurring at colour and intensity levels as well as to evaluate the efficiency of image normalisation methods in correcting them. We applied our methodology to test different methods of image normalisation ba

SUBMITTER: Van Eycke YR 

PROVIDER: S-EPMC5318955 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature