Deep Illumina sequencing reveals conserved and novel microRNAs in grass carp in response to grass carp reovirus infection.

ABSTRACT:

Background

The grass carp hemorrhagic disease caused by the grass carp reovirus (GCRV) is a major disease that hampers the development of grass carp aquaculture. The mechanism underlying GCRV pathogenesis and hemorrhagic symptoms is still unknown. MicroRNAs (miRNAs) are key regulators involved in various biological processes. The aim of this study was to identify conserved and novel miRNAs in grass carp in response to GCRV infection, as well as attempt to reveal the mechanism underlying GCRV pathogenesis and hemorrhagic symptoms.

Results

Grass carp were infected with GCRV, and spleen samples were collected at 0 (control), 1, 3, 5, 7, and 9 days post-infection (dpi). These samples were used to construct and sequence small RNA libraries. A total of 1208 miRNAs were identified,

SUBMITTER: He L 

PROVIDER: S-EPMC5319172 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature