Identification of circo-like virus-Brazil genomic sequences in raw sewage from the metropolitan area of Sao Paulo: evidence of circulation two and three years after the first detection.

ABSTRACT:

Background

Two novel viruses named circo-like virus-Brazil (CLV-BR) hs1 and hs2 were previously discovered in a Brazilian human fecal sample through metagenomics. CLV-BR hs1 and hs2 possess a small circular DNA genome encoding a replication initiator protein (Rep), and the two genomes exhibit 92% nucleotide identity with each other. Phylogenetic analysis based on the Rep protein showed that CLV-BRs do not cluster with circoviruses, nanoviruses, geminiviruses or cycloviruses.

Objective

The aim of this study was to search for CLV-BR genomes in sewage and reclaimed water samples from the metropolitan area of São Paulo, Brazil, to verify whether the first detection of these viruses was an isolated finding.

Methods

Sewage and reclaimed water samples collected concomitantly

SUBMITTER: Castrignano SB 

PROVIDER: S-EPMC5319365 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature