Identification of novel cancer therapeutic targets using a designed and pooled shRNA library screen.

ABSTRACT: Targeted cancer therapeutics aim to exploit tumor-specific, genetic vulnerabilities specifically affecting neoplastic cells without similarly affecting normal cells. Here we performed sequencing-based screening of an shRNA library on a panel of cancer cells of different origins as well as normal cells. The shRNA library was designed to target a subset of genes previously identified using a whole genome screening approach. This focused shRNA library was infected into cells followed by analysis of enrichment and depletion of the shRNAs over the course of cell proliferation. We developed a bootstrap likelihood ratio test for the interpretation of the effects of multiple shRNAs over multiple cell line passages. Our analysis identified 44 genes whose depletion preferentially inhibited the growt

SUBMITTER: Oliver D 

PROVIDER: S-EPMC5320502 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature