The general PTS component HPr determines the preference for glucose over mannitol.

ABSTRACT: Preferential sugar utilization is a widespread phenomenon in biological systems. Glucose is usually the most preferred carbon source in various organisms, especially in bacteria where it is taken up via the phosphoenolpyruvate:sugar phosphotransferase system (PTS). The currently proposed model for glucose preference over non-PTS sugars in enteric bacteria including E. coli is strictly dependent on the phosphorylation state of the glucose-specific PTS component, enzyme IIA^Glc (EIIA^Glc). However, the mechanism of the preference among PTS sugars is largely unknown in Gram-negative bacteria. Here, we show that glucose preference over another PTS sugar, mannitol, is absolutely dependent on the general PTS component HPr, but not on EIIA^Glc, in E. coli. Dephosphor

SUBMITTER: Choe M 

PROVIDER: S-EPMC5320558 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature