Molecular and biochemical characterization of the NS1 protein of non-cultured influenza B virus strains circulating in Singapore.

ABSTRACT: In this study we compared the NS1 protein of Influenza B/Lee/40 and several non-cultured Influenza B virus clinical strains detected in Singapore. In B/Lee/40 virus-infected cells and in cells expressing the recombinant B/Lee/40 NS1 protein a full-length 35 kDa NS1 protein and a 23 kDa NS1 protein species (p23) were detected. Mutational analysis of the NS1 gene indicated that p23 was generated by a novel cleavage event within the linker domain between an aspartic acid and proline at amino acid residues at positions 92 and 93 respectively (DP_92-93), and that p23 contained the first 92 amino acids of the NS1 protein. Sequence analysis of the Singapore strains indicated the presence of either DP_92-93 or NP_92-93 in the NS1 protein, but protein expression analys

SUBMITTER: Jumat MR 

PROVIDER: S-EPMC5320597 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature