Robust high-throughput prokaryote de novo assembly and improvement pipeline for Illumina data.

ABSTRACT: The rapidly reducing cost of bacterial genome sequencing has lead to its routine use in large-scale microbial analysis. Though mapping approaches can be used to find differences relative to the reference, many bacteria are subject to constant evolutionary pressures resulting in events such as the loss and gain of mobile genetic elements, horizontal gene transfer through recombination and genomic rearrangements. De novo assembly is the reconstruction of the underlying genome sequence, an essential step to understanding bacterial genome diversity. Here we present a high-throughput bacterial assembly and improvement pipeline that has been used to generate nearly 20 000 annotated draft genome assemblies in public databases. We demonstrate its performance on a public data set of 9404 gen

SUBMITTER: Page AJ 

PROVIDER: S-EPMC5320598 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature