A Modular Method for the High-Yield Synthesis of Site-Specific Protein-Polymer Therapeutics.

ABSTRACT: A versatile method is described to engineer precisely defined protein/peptide-polymer therapeutics by a modular approach that consists of three steps: 1) fusion of a protein/peptide of interest with an elastin-like polypeptide that enables facile purification and high yields; 2) installation of a clickable group at the C terminus of the recombinant protein/peptide with almost complete conversion by enzyme-mediated ligation; and 3) attachment of a polymer by a click reaction with near-quantitative conversion. We demonstrate that this modular approach is applicable to various protein/peptide drugs and used it to conjugate them to structurally diverse water-soluble polymers that prolong the plasma circulation duration of these proteins. The protein/peptide-polymer conjugates exhibited signifi

SUBMITTER: Pang Y 

PROVIDER: S-EPMC5320947 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature