Trifunctional lipid probes for comprehensive studies of single lipid species in living cells.

ABSTRACT: Lipid-mediated signaling events regulate many cellular processes. Investigations of the complex underlying mechanisms are difficult because several different methods need to be used under varying conditions. Here we introduce multifunctional lipid derivatives to study lipid metabolism, lipid-protein interactions, and intracellular lipid localization with a single tool per target lipid. The probes are equipped with two photoreactive groups to allow photoliberation (uncaging) and photo-cross-linking in a sequential manner, as well as a click-handle for subsequent functionalization. We demonstrate the versatility of the design for the signaling lipids sphingosine and diacylglycerol; uncaging of the probe for these two species triggered calcium signaling and intracellular protein translocation

SUBMITTER: Hoglinger D 

PROVIDER: S-EPMC5320957 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature