Elucidation of roles for vitamin B₁₂ in regulation of folate, ubiquinone, and methionine metabolism.

ABSTRACT: Only a small fraction of vitamin B₁₂-requiring organisms are able to synthesize B₁₂ de novo, making it a common commodity in microbial communities. Initially recognized as an enzyme cofactor of a few enzymes, recent studies have revealed additional B₁₂-binding enzymes and regulatory roles for B₁₂ Here we report the development and use of a B₁₂-based chemical probe to identify B₁₂-binding proteins in a nonphototrophic B₁₂-producing bacterium. Two unexpected discoveries resulted from this study. First, we identified a light-sensing B₁₂-binding transcriptional regulator and demonstrated that it controls folate and ubiquinone biosynthesis. Second, our probe captured proteins involved in folate, methionine,

SUBMITTER: Romine MF 

PROVIDER: S-EPMC5320969 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature