Lack of mature lymphocytes results in obese but metabolically healthy mice when fed a high-fat diet.

ABSTRACT:

Background/objectives

Obesity is characterized by chronic inflammation and immune dysregulation, as well as insulin resistance, but the link between obesity and adaptive immunity remains to be fully studied.

Methods

To elucidate the role of adaptive immunity on body composition, glucose homeostasis and inflammation, recombination-activating gene 1 knockout (Rag1-/-) mice, without mature T-lymphocytes or B-lymphocytes, were maintained on a low- or high-fat diet (LFD and HFD, respectively) for 11 weeks.

Results

Rag1-/- mice fed HFD gained significantly more weight and had increased body fat compared with wild type. Downregulation of energy expenditure as well as brown fat uncoupling protein UCP-1 and UCP-3 gene expression were noticed in HFD-fed Rag1-/- mice compared wi

SUBMITTER: Liu X 

PROVIDER: S-EPMC5321118 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature