Measuring Oxygen Consumption Rate in Caenorhabditis elegans.

ABSTRACT: The rate of oxygen consumption is a vital marker indicating cellular function during lifetime under normal or metabolically challenged conditions. It is used broadly to study mitochondrial function (Artal-Sanz and Tavernarakis, 2009; Palikaras et al., 2015; Ryu et al., 2016) or investigate factors mediating the switch from oxidative phosphorylation to aerobic glycolysis (Chen et al., 2015; Vander Heiden et al., 2009). In this protocol, we describe a method for the determination of oxygen consumption rates in the nematode Caenorhabditis elegans.

SUBMITTER: Palikaras K 

PROVIDER: S-EPMC5321503 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature