Layer-specific optogenetic activation of pyramidal neurons causes beta-gamma entrainment of neonatal networks.

ABSTRACT: Coordinated activity patterns in the developing brain may contribute to the wiring of neuronal circuits underlying future behavioural requirements. However, causal evidence for this hypothesis has been difficult to obtain owing to the absence of tools for selective manipulation of oscillations during early development. We established a protocol that combines optogenetics with electrophysiological recordings from neonatal mice in vivo to elucidate the substrate of early network oscillations in the prefrontal cortex. We show that light-induced activation of layer II/III pyramidal neurons that are transfected by in utero electroporation with a high-efficiency channelrhodopsin drives frequency-specific spiking and boosts network oscillations within beta-gamma frequency range. By contrast, acti

SUBMITTER: Bitzenhofer SH 

PROVIDER: S-EPMC5321724 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature