Impact of genetic background and experimental reproducibility on identifying chemical compounds with robust longevity effects.

ABSTRACT: Limiting the debilitating consequences of ageing is a major medical challenge of our time. Robust pharmacological interventions that promote healthy ageing across diverse genetic backgrounds may engage conserved longevity pathways. Here we report results from the Caenorhabditis Intervention Testing Program in assessing longevity variation across 22 Caenorhabditis strains spanning 3 species, using multiple replicates collected across three independent laboratories. Reproducibility between test sites is high, whereas individual trial reproducibility is relatively low. Of ten pro-longevity chemicals tested, six significantly extend lifespan in at least one strain. Three reported dietary restriction mimetics are mainly effective across C. elegans strains, indicating species and strain-specific

SUBMITTER: Lucanic M 

PROVIDER: S-EPMC5321775 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature