System-based proteomic and metabonomic analysis of the Df(16)A^+/- mouse identifies potential miR-185 targets and molecular pathway alterations.

ABSTRACT: Deletions on chromosome 22q11.2 are a strong genetic risk factor for development of schizophrenia and cognitive dysfunction. We employed shotgun liquid chromatography-mass spectrometry (LC-MS) proteomic and metabonomic profiling approaches on prefrontal cortex (PFC) and hippocampal (HPC) tissue from Df(16)A^+/- mice, a model of the 22q11.2 deletion syndrome. Proteomic results were compared with previous transcriptomic profiling studies of the same brain regions. The aim was to investigate how the combined effect of the 22q11.2 deletion and the corresponding miRNA dysregulation affects the cell biology at the systems level. The proteomic brain profiling analysis revealed PFC and HPC changes in various molecular pathways associated with chromatin remodelling and RNA transcription,

SUBMITTER: Wesseling H 

PROVIDER: S-EPMC5322275 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature