Simultaneous dual-color fluorescence lifetime imaging with novel red-shifted fluorescent proteins.

ABSTRACT: We describe a red-shifted fluorescence resonance energy transfer (FRET) pair optimized for dual-color fluorescence lifetime imaging (FLIM). This pair utilizes a newly developed FRET donor, monomeric cyan-excitable red fluorescent protein (mCyRFP1), which has a large Stokes shift and a monoexponential fluorescence lifetime decay. When used together with EGFP-based biosensors, the new pair enables simultaneous imaging of the activities of two signaling molecules in single dendritic spines undergoing structural plasticity.

SUBMITTER: Laviv T 

PROVIDER: S-EPMC5322478 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature