Passenger mutations and aberrant gene expression in congenic tissue plasminogen activator-deficient mouse strains.

ABSTRACT:

Unlabelled

Essentials C57BL/6J-tissue plasminogen activator (tPA)-deficient mice are widely used to study tPA function. Congenic C57BL/6J-tPA-deficient mice harbor large 129-derived chromosomal segments. The 129-derived chromosomal segments contain gene mutations that may confound data interpretation. Passenger mutation-free isogenic tPA-deficient mice were generated for study of tPA function.

Summary

Background The ability to generate defined null mutations in mice revolutionized the analysis of gene function in mammals. However, gene-deficient mice generated by using 129-derived embryonic stem cells may carry large segments of 129 DNA, even when extensively backcrossed to reference strains, such as C57BL/6J, and this may confound interpretation of experiments performed in

SUBMITTER: Szabo R 

PROVIDER: S-EPMC5322813 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature