Intrinsic Astrocyte Heterogeneity Influences Tumor Growth in Glioma Mouse Models.

ABSTRACT: The influence of cellular origin on glioma pathogenesis remains elusive. We previously showed that mutations inactivating Rb and Pten and activating Kras transform astrocytes and induce tumorigenesis throughout the adult mouse brain. However, it remained unclear whether astrocyte subpopulations were susceptible to these mutations. We therefore used genetic lineage tracing and fate mapping in adult conditional, inducible genetically engineered mice to monitor transformation of glial fibrillary acidic protein (GFAP) and glutamate aspartate transporter (GLAST) astrocytes and immunofluorescence to monitor cellular composition of the tumor microenvironment over time. Because considerable regional heterogeneity exists among astrocytes, we also examined the influence of brain region on tumor grow

SUBMITTER: Irvin DM 

PROVIDER: S-EPMC5322824 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature