A Simple and Sensitive High-Content Assay for the Characterization of Antiproliferative Therapeutic Antibodies.

ABSTRACT: Monoclonal antibodies (mAbs) have become a central class of therapeutic agents in particular as antiproliferative compounds. Their often complex modes of action require sensitive assays during early, functional characterization. Current cell-based proliferation assays often detect metabolites that are indicative of metabolic activity but do not directly account for cell proliferation. Measuring DNA replication by incorporation of base analogues such as 5-bromo-2'-deoxyuridine (BrdU) fills this analytical gap but was previously restricted to bulk effect characterization in enzyme-linked immunosorbent assay formats. Here, we describe a cell-based assay format for the characterization of antiproliferative mAbs regarding potency and mode of action in a single experiment. The assay makes use of

SUBMITTER: Stengl A 

PROVIDER: S-EPMC5322830 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature